On the Reaction Mechanism of Yeast Glutathione Reductase
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چکیده
منابع مشابه
On the reaction mechanism of yeast glutathione reductase.
Glutathione reductase has been purified from a number of sources, Escherichiu coli (l), yeast (2, 3), peas (4), rat tissues (5, 6), and erythrocytes (7). In all of the cases, the enzyme appears to be a flavoprotein, containing flavin adenine dinucleotide as prosthetic group (8). In view of the fact that the over-all reaction catalyzed by the enzyme, GSSG + TPNH + H+ & 2 GSH + TPN+, is formally ...
متن کاملThe catalytic mechanism of glutathione reductase as derived from x-ray diffraction analyses of reaction intermediates.
The mode of binding of NADPH and oxidized glutathione to the flavoenzyme glutathione reductase has been determined by x-ray crystallography. Furthermore, two intermediates of the reaction have been produced in the crystal and have been structurally elucidated. All these analyses were done at 0.3 nm resolution. The results allow the stereochemical description of the mechanism of the enzyme. The ...
متن کاملGlutathione reductase from bakers' yeast and beef liver.
The reduction of glutathione by a heat-labile system in liver was discovered by Hopkins and Elliott (1). Later Mann (2) obtained a soluble enzyme preparation from liver which required, for the reduction process, a cofactor and glucose as hydrogen donor. Meldrum and Tarr (3) found that oxidized glutathione was reduced by rat blood and by yeast and demonstrated the function of TPNl as a cofactor ...
متن کاملRegulatory mechanism of glutathione reductase activity in human red cells.
The mechanism by which glutathione reductase (GR) activity is regulated in relation to fiavin metabolism was studied in red cells of normal adults, cord blood, and patients with severe metabolic disorders using spectrophotometnc, fluorometnc, and radiochemical methods. The increased activity of GR in red cells of adult patients with severe hepatic cirrhosis, chronic uremia, and glucose-6-phosph...
متن کاملsurvey on the rule of the due & hindering relying on the sheikh ansaris ideas
قاعده مقتضی و مانع در متون فقهی کم و بیش مستند احکام قرار گرفته و مورد مناقشه فقهاء و اصولیین می باشد و مشهور معتقند مقتضی و مانع، قاعده نیست بلکه یکی از مسائل ذیل استصحاب است لذا نگارنده بر آن شد تا پیرامون این قاعده پژوهش جامعی انجام دهد. به عقیده ما مقتضی دارای حیثیت مستقلی است و هر گاه می گوییم مقتضی احراز شد یعنی با ماهیت مستقل خودش محرز گشته و قطعا اقتضاء خود را خواهد داشت مانند نکاح که ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1965
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)97085-7